What does the commonly used DCF test for oxidative stress really show?

What does the commonly used DCF test for oxidative stress really show?

H(2)DCF-DA (dihydrodichlorofluorescein diacetate) is widely used to evaluate 'cellular oxidative stress'. After passing through the plasma membrane, this lipophilic and non-fluorescent compound is de-esterified to a hydrophilic alcohol [H(2)DCF (dihydrodichlorofluorescein)] that may be oxidized to fluorescent DCF (2',7'-dichlorofluorescein) by a process usually considered to involve ROS (reacti...

The Swimmer's view: does it really show what it is supposed to show? A retrospective study

BACKGROUND One of the basic principles in the primary survey of a trauma patient is immobilisation of the cervical spine till cleared of any injury. Lateral cervical spine radiograph is one of the important initial radiographic assessments. More than often additional radiographs like the Swimmer's view are necessary for adequate visualisation of the cervical spine. How good is the Swimmer's vie...

What Does the Quantitative Research Literature Really Show about Teaching Methods?

What Is Stress? Dose-Response Effects in Commonly Used in Vitro Stress Assays.

In vitro stress assays are commonly used to study the responses of plants to abiotic stress and to assess stress tolerance. A literature review reveals that most studies use very high stress levels and measure criteria such as germination, plant survival, or the development of visual symptoms such as bleaching. However, we show that these parameters are indicators of very severe stress, and suc...

What Does PLIER Really Do?

MOTIVATION Our goal was to understand why the PLIER algorithm performs so well given its derivation is based on a biologically implausible assumption. RESULTS In spite of a non-intuitive assumption regarding the PM and MM errors made as part of the derivation for PLIER, the resulting probe level error function does capture the key characteristics of the ideal error function, assuming MM probe...